The Cofactor Requirements of Sarcosine Oxidase
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چکیده
منابع مشابه
The cofactor requirements of sarcosine oxidase.
It has been established that the enzyme, sarcosine oxidase, found in the mitochondria of rat liver and elsewhere (I), catalyzes the following over-all reaction (2, 3) : sarcosine + $02 = glycine + serine + HCHO. The cofactor requirements of this reaction have not been established, although Mackenzie (1) has mentioned unpublished results which seemed to indicate that a folic acid deficiency in r...
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Introduction The concentration of serum creatinine is widely used as an indicator of renal function. Sarcosine oxidase (SO) has been used in the enzymatic determination of creatinine in the clinical laboratory. SO has been isolated from various bacterial strains. It was first purified from Corynebacterium sp. U-96 (SO-U96) and shown to be a heterotetrameric enzyme. SO-U96 is a complex, bifuncti...
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GO (galactose oxidase; E.C. 1.1.3.9) is a monomeric 68 kDa enzyme that contains a single copper ion and an amino acid-derived cofactor. The enzyme is produced by the filamentous fungus Fusarium graminearum as an extracellular enzyme. The enzyme has been extensively studied by structural, spectroscopic, kinetic and mutational approaches that have provided insight into the catalytic mechanism of ...
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A "sarcosine oxidase" was prepared from a creatinine-decomposing strain of Pseudomonas aeruginosa. The enzyme is inactivated by drying, lyophilization, and dialysis against distilled water. No dialyzable cofactor was found. Optimal activity of the enzyme is reached at pH 7.8. Enzyme activity is directly proportional to enzyme concentration and also to substrate concentration up to the point of ...
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The reduction of nitrite to nitric oxide has been shown to require DPN or TPN, FAD or FMN,’ copper, and iron as electron carriers. This was determined by the stimulation of nitrite disappearance as well as by increased nitric oxide formation. Evidence was also presented indicating that the reduction of NO to Nz was stimulated by the same cofactors. The evidence was based on the stimulation of N...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1958
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)68060-9